Shiny Maity – @shiny_maity
Water dynamics near AsLOV2’s surface decrease upon light activation, suggesting expulsion of wrap water and leaving a tightly bound hydration shell. Under high pressure, AsLOV2 shifts from folded to unfolded states, mimicking light’s effect. 17O NMR reveals distinct water populations. We aim to identify light’s impact on these water populations.
Bijaylaxmi Patra – @BijaylaxmiNMR
Glycosaminoglycans (GAGs) and protein interactions are essential in numerous biochemical processes involving ion-pair interactions. However, there is no evidence of this, in native cartilage. Using dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR, we detected these interactions, revealing charge-pair salt-bridges and potential hydrogen bonding, offering new structural insights.
Mayra Paola Oquist Phillips – @oquist_paola
Glycoprotein 120 (gp120) shields the HIV virus using high mannose glycans, which are difficult to analyze structurally due to their high isochronicity causing NMR signal collapse. Novel NMR methods, including paramagnetic probes, now enable detailed study of gp120 and its interaction with the neutralizing antibody 2G12.
Orr Lusky – @ItIsLusk
We present a robust method using ssNMR to characterise the protein dynamics in an intact virus using pseudo 3D experiments. We show an automated protocol to quantify site-specific order parameters in the backbone, and demonstrate it on the fd-Y21M bacteriophage.
Alexandra Gloria – @_AGloria
Feedlot cattle are crucial to Australia’s livestock industry, supplying beef for domestic and export markets. High summer temperatures and humidity increase heat stress vulnerability, impacting growth, productivity, and welfare. We use NMR-based metabolomics and clinical biochemistry to understand the metabolic effects of heat stress to help mitigate its impact.
Somnath Mondal – @somomsom
The picornaviral 3C(D) protein is a vital protease that cleaves viral polyproteins and host cell defense proteins, interacting with replication elements to control replication and translation. Its interaction with RNA, causing liquid-liquid phase separation, could influence viral apoptosis and infection in the host cells.
Julia Pakuła – @x_yuliya_x
Emerging as a promising area in anticancer research, noncanonical DNA structures such as G-quadruplexes are studied using NMR spectroscopy. NOESY spectra reveals crucial interactions between ligands and G-quadruplexes, helping to understand non-covalent binding mechanisms. This knowledge is essential for rational drug design targeting these structures.
Sarah Walsh – @SarahW29329
Wolbachia, a common endosymbiont of insects, exerts significant antiviral effects. This phenomenon is valuable in reducing the spread of arboviruses, such as dengue. Employing NMR metabolomics, we uncover that in virus-infected Drosophila melanogaster Wolbachia drives host metabolism in a way that inhibits RNA virus replication both directly and indirectly.
Yara Lins Rocha – @YaraLRocha
Enzymes belonging to the Carbapenem-Hydrolyzing Class D β-lactamases (CHDL’s) confer bacterial resistance to β-lactam antibiotics and are often associated with outbreaks involving the pathogenic bacteria Acinetobacter baumannii. We report the inhibition of CHDL OXA-143 by the reaction product with meropenem, a carbapenem antibiotic, in addition to describing its effect on protein structure and dynamics. Kinetic 1H-NMR experiments were performed to assess how hMER interferes with the catalytic efficiency of the enzyme against ampicillin and meropenem itself, indicating a clear inhibition. Chemical shift perturbation (CSP) was performed using 15N-HSQC and 15N-TROSY titration experiments. The results indicate that hMER acts as a competitive inhibitor blocking the active site and decreasing the dynamics of essential motifs around the binding site.
Rajesh Kumar Reddy Sannapureddi – @RR_Sannapureddi
Parallel G-quadruplexes formed by guanine rich sequences are ubiquitously formed in promoter, telomere regions. Structural and dynamic features associated with them is presented in details from structure survey, solution state NMR studies and MD simulations. Impact of covalently attached duplex on parallel quadruplex discerned from NMR studies are presented.
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