Orr Simon Lusky – @ItIsLusk
We present a new solid state NMR method we developed for studying biological macromolecules called ‘Pulse induced resonance with angular dependent total enhancement’ (PIRATE). We demonstrate a new resonance condition, and the application on finding hydrogen bond patterns in intact viral RNA harvested directly from the MS2 bacteriophage.
Brad D. Price – @braddprice
An in-depth functional understanding of proteins requires a technique for tracking their movement in real time. We present high-field, (tr)EPR ‘filming’ of room-temperature protein motion as progress toward real-time distance tracking in lifelike environments.
Xinyao Xiang – @xxiang2022
The nanoparticle-assisted NMR spin relaxation (NASR) method measures the enhancement in transverse spin relaxation in the presence of slowly tumbling nanoparticles. It uncovers protein internal motions on the previously unobservable nanosecond to microsecond regime. We recently extended our NASR method to methyl-side chains using 2H or 13C spin relaxation.
Soumendu Boral – @BoralSoumendu
Inteins perform protein splicing reaction. We solved solution NMR structure (PDB code: 7CFV) of a 136-residue DnaX mini-intein. The NMR structural ensemble has backbone and heavy atom RMSD of 0.27 and 0.52 Å, respectively. NMR-based HX and 15N-relaxation experiments showed the presence of a very stable core and conserved motions.
Sumit Mishra – @sumitamu
Momordica charantia is a medicinal plant which is widely used to treat several diseases. We have identified the phytosterols in the pericarp, skin and seeds parts of M. charantia fruit via NMR spectroscopy. Multivariate statistical analysis showed a clustering of the metabolic profiles of seeds and pericarp, and their clear separation from the metabolic profile of the skin.
Chris Waudby – @chris_waudby
Julia is a modern language optimised for scientific computation. To facilitate the use of Julia by the NMR community, here we introduce NMRTools.jl, an open source package providing an interface to import and visualise NMR data. Interested users are welcomed to contribute to the development of this ongoing project.
Ángela Serrano Sánchez – @Angelasrno99
We have optimised a new, simple methodology to assess membrane permeability of drugs making use of the paramagnetic relaxation enhancement (PRE) effect in lipid vesicles that is applicable to any type of cell membrane. Using this assay, we have been able to find important differences in the permeability of new families of antimicrobial compounds, providing unique information for the further development of this family of compounds. In addition, we have now expanded this assay to live cells making use of a NMR-coupled bioreactor. Using this set up, we are to monitor both the drug permeation into live cells, as well as changes in the bacterial metabolism as a result of the antimicrobial agent added.
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