Unveiling Charge-Pair Salt-Bridge Interaction Between GAGs and Collagen Protein in Cartilage: Atomic Evidence from DNP-Enhanced ssNMR at Natural Isotopic Abundance

Bijaylaxmi Patra – @BijaylaxmiNMR

Excited to delve into 15N-13C and 13C-13C correlations in natural abundance ! Watch this video to learn how we unveil interactions between GAGs and collagen protein via DNP-enhanced ssNMR.#GlobalnmrOC2024 @NeerajS13902691 @DNPFred @NationalMagLab @cbmrofficial pic.twitter.com/IZJryBtJfs

— Bijaylaxmi Patra (@BijaylaxmiNMR) July 17, 2024

Glycosaminoglycans (GAGs) and protein interactions are essential in numerous biochemical processes involving ion-pair interactions. However, there is no evidence of this, in native cartilage. Using dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR, we detected these interactions, revealing charge-pair salt-bridges and potential hydrogen bonding, offering new structural insights.