Integrative Structural Biology: Challenges and Opportunities for MR
During the 93rd session of the Global NMR Discussion Meetings held on December 3rd, 2024, via Zoom, Prof. Tatyana Polenova from the University of Delaware, USA, gave a talk on the topic "Integrative Structural Biology of Protein Assemblies: Challenges and Opportunities for Magnetic Resonance". The recording serves as a tutorial.
Abstract: I will present recent developments in MAS NMR techniques for atomic-resolution structural analysis of large biological assemblies. Drawing on studies of diverse systems from our lab, including the kinesin motor domain with polymerized microtubules, cofilin bound to filamentous actin, and HIV-1 protein assemblies interacting with small-molecule maturation inhibitors, I will illustrate how with MAS NMR, structural and dynamic details, as well as drug interactions were uncovered, that are inaccessible using other approaches. Additionally, I will discuss the benefits of combining MAS NMR with medium-resolution cryo-EM and MD simulations to enhance structural understanding, and address the current challenges and future directions for magnetic resonance in integrative structural biology.
Find out more about Prof. Tatyana Polenova's research: https://www.udel.edu/faculty-staff/experts/tatyana-polenova/