Videos
Integrative Structural Biology: Challenges and Opportunities for MR
During the 93rd session of the Global NMR Discussion Meetings held on December 3rd, 2024, via Zoom, Prof. Tatyana Polenova from the University of Delaware, USA, gave a talk on the topic "Integrative Structural Biology of Protein Assemblies: Challenges and Opportunities for Magnetic Resonance". The recording serves as a tutorial.
Abstract: I will present recent developments in MAS NMR techniques for atomic-resolution structural analysis of large biological assemblies. Drawing on studies of diverse systems from our lab, including the kinesin motor domain with polymerized microtubules, cofilin bound to filamentous actin, and HIV-1 protein assemblies interacting with small-molecule maturation inhibitors, I will illustrate how with MAS NMR, structural and dynamic details, as well as drug interactions were uncovered, that are inaccessible using other approaches. Additionally, I will discuss the benefits of combining MAS NMR with medium-resolution cryo-EM and MD simulations to enhance structural understanding, and address the current challenges and future directions for magnetic resonance in integrative structural biology.
Find out more about Prof. Tatyana Polenova's research: https://www.udel.edu/faculty-staff/experts/tatyana-polenova/
NMR approaches for intrinsically disordered proteins
During the 91st session of the Global NMR Discussion Meetings held on November 5th, 2024, via Zoom, Prof. Julie Forman-Kay from the University of Toronto & SickKids Research Institute, Canada, gave a talk on the topic "NMR approaches for intrinsically disordered proteins". The recording serves as a tutorial.
Abstract: NMR is a powerful tool for obtaining site-specific information about dynamic systems, including intrinsically disordered proteins and protein regions (IDPs/IDRs) which do not adopt unique, stable folded structures. While computational approaches are increasingly powerful for stable proteins and protein domains, there is a huge need for experimental information about IDPs, IDRs and the highly dynamic complexes that they often make with other disordered proteins, folded domains and other biomolecules. The lecture will highlight examples of NMR studies of IDPs and their dynamic complexes, including condensed state models of those found in biomolecular condensates.
Find out more about Prof. Julie Forman-Kay's research: https://biochemistry.utoronto.ca/person/julie-d-forman-kay/
Understanding DNP : Insights from Average Hamiltonian Theory
During the 90th session of the Global NMR Discussion Meetings held on October 22nd, 2024 via Zoom, Prof. Sheetal Kumar Jain from the Indian Institute of Science, Bengaluru, India, gave a talk on the topic "Understanding Dynamic Nuclear Polarization: Insights from Average Hamiltonian Theory". The recording serves as a tutorial.
Abstract: Average Hamiltonian Theory (AHT) effectively explains the mechanisms, efficiencies, and optimal conditions for solid-state NMR pulse sequences. In Dynamic Nuclear Polarization (DNP), AHT describes how electron-nuclear spin couplings drive polarization transfer under microwave irradiation. By simplifying spin dynamics, AHT offers insights into polarization mechanisms like the solid effect, cross effect, and pulsed DNP, helping predict conditions to maximize nuclear spin polarization and enhance NMR signal sensitivity.
Find out more about Prof. Sheetal Kumar Jain's research: https://sscu.iisc.ac.in/sheetal-kumar-jain/
Low-Field Magnetic Resonance for Detection and Other Applications
During the 89th session of the Global NMR Discussion Meetings held on October 8th, 2024 via Zoom, Dr. Adam Altenhof from the Los Alamos National Laboratory, New Mexico, US, gave a talk on the topic "Low-Field Magnetic Resonance for Detection and Other Applications". The recording serves as a tutorial
Abstract: Low-field NMR can offer better resolution than at high-field. Extremely homogeneous B0 fields and low susceptibility broadening results in signal lifetimes around 1 – 10’s of seconds. Earth’s field NMR and nuclear quadrupole resonance (NQR) both offer low cost, high portability, and applications for detection of illicit materials, including fentanyl.
Find out more about Dr. Adam Altenhof's work: https://orcid.org/0000-0002-8095-6373
Electron Paramagnetic Resonance Advancing MAS-DNP
During the 88th session of the Global NMR Discussion Meetings held on October 1st, 2024 via Zoom, Dr. Frédéric Mentink-Vigier from the National High Magnetic Field Laboratory, Florida, US gave a talk on the topic "Electron Paramagnetic Resonance Advancing Magic Angle Spinning Dynamic Nuclear polarization". The recording serves as a tutorial.
Abstract: Electron Paramagnetic Resonance (EPR) is crucial for analyzing paramagnetic species used in Dynamic Nuclear Polarization (DNP). This overview covers EPR fundamentals and its application to studying biradicals, which are key for high-field Magic Angle Spinning (MAS) DNP.
Find out more about Dr. Frédéric Mentink-Vigier's work: https://nationalmaglab.org/staff/?name=FredericMentink-vigier
Parahydrogen-enhanced benchtop NMR spectroscopy
During the 87th session of the Global NMR Discussion Meetings held on June 4th, 2024 via Zoom, Dr. Meghan Halse from the University of York, UK gave a talk on the topic "Parahydrogen-enhanced benchtop NMR spectroscopy". The recording serves as a tutorial.
Abstract: Benchtop NMR spectrometers, due to their portability and affordability, have the potential to transform the accessibility of NMR spectroscopy. Hyperpolarisation can break the link between NMR sensitivity and magnetic field strength, overcoming a key limitation of low-field benchtop NMR spectrometers. This presentation will explain the fundamentals of parahydrogen hyperpolarisation and explore recent developments in its use with benchtop NMR detection, including progress towards analytical applications.
Dr. Meghan Halse's research: https://www.york.ac.uk/chemistry/people/mhalse/
Gradient Pulses On Moving Spins
During the 86th session of the Global NMR Discussion Meetings held on May 21st, 2024 via Zoom, Dr. Jean Nicolas Dumez from the University of Nantes, France gave a talk on the topic "Gradient pulses on moving spins: flow NMR methods for reaction monitoring". The recording serves as a tutorial.
Abstract: Flow NMR is a powerful approach to monitor chemical reactions, with applications in fields such as catalysis and polymer science. The sample flow makes it necessary to revisit some of the core concepts of NMR pulse sequences. This presentation will describe recent developments of fast multidimensional NMR methods that are applicable in continuous flow, and the underlying spin dynamics concepts.
Dr. Dumez's research: https://www.univ-nantes.fr/jean-nicolas-dumez
Long-loved nuclear singlet spin order and its applications
During the 84th session of the Global NMR Discussion Meetings held on April 30th, 2024 via Zoom, Prof. Giuseppe Pileio from the University of Southampton, UK gave a talk on the topic "Long-loved nuclear singlet spin order and its applications". The recording serves as a tutorial.
Abstract: Nuclear singlet spin order is the population difference between the singlet and triplet states in a system of two coupled spin-1/2 nuclei. This form of order is long-lived, silent and accessible on demand. For almost two decades, my research activities were focused at exploiting these three main properties of nuclear spin order to develop new applications in NMR and MRI. In this talk, I will introduce the concept and the main features of singlet order as well as the tools developed for its manipulations. I will then show how we are using this form of order to enhance several NMR and MRI techniques for the long-term storage of hyperpolarisation, to obtain a new form of contrast in MRI, for the measurements of slow diffusion and flow, or for the determination of structural features of porous media such as tortuosity and structural anisotropy through singlet-assisted diffusion NMR.
Prof. Pileio's research: https://generic.wordpress.soton.ac.uk/gpgroup/
Hyperpolarized Xe NMR in Molecular Cavities Study and MR Imaging
During the 83rd session of the Global NMR Discussion Meetings held on March 26th, 2024 via Zoom, Prof. Leif Schröder from the German Cancer Research Center gave a talk on the topic "Hyperpolarized Xenon NMR for Exploring Molecular Host Cavities and Advancing MR Imaging". The recording serves as a tutorial.
Abstract: The large chemical shift range of Xe-129 NMR makes this nucleus a valuable probe in spectroscopy and biomedical imaging. Hyperpolarized Xe can be used also as dissolved noble gas to provide 10’000-fold improved sensitivity over extended periods of time. In combination with saturation transfer techniques, reversibly bound hyperpolarized xenon gives insights into exchange kinetics of various host-guest complexes. This talk will give an overview of studies investigating the affinity of dissolved Xe for various hosts like biogenic hollow protein structures with attoliter volumes or synthetic hosts for trapping individual Xe atoms. Both strategies find applications in the spectroscopic investigation of exchange kinetics of host-guest systems and in the design of ultra-sensitive magnetic resonance imaging agents.
Prof. Schroeder's research: https://www.dkfz.de/en/translationale-molekulare-bildgebung/index.php
NMR Metabolomics in Drug Discovery and Disease Diagnosis
During the 82nd session of the Global NMR Discussion Meetings held on March 12th, 2024 via Zoom, Prof. Robert Powers from the University of Nebraska-Lincoln, United States, gave a talk on the topic "The Application of NMR Metabolomics to Drug Discovery and Disease Diagnosis". The recording serves as a tutorial.
Abstract: NMR-based metabolomics has benefited a variety of fields including drug discovery and disease diagnosis. Metabolomics is technically very challenging and requires expertise in a diversity of scientific areas. In this regard, this tutorial will discuss best practices for sample preparation and handling, data collection and analysis, and statistical modeling and interpretation to achieve biologically and clinically significant results.
Prof. Robert Powers' research group website and bio: https://chem.unl.edu/robert-powers
Advances in the Computation of NMR Interactions in Materials
During the 81st session of the Global NMR Discussion Meetings held on February 27th, 2024 via Zoom, Dr. Sean T. Holmes from the Florida State University, United States, gave a talk on the topic "Advances in the computation of NMR interactions in materials". The recording serves as a tutorial.
Abstract: This lecture will discuss recent advances in the computation of NMR interactions in materials using density functional theory methods. These include methods for accounting for intermolecular interactions, the role of relativistic effects (especially for heavy atoms), and the choice of density functional approximation. Applications to elements from across the Periodic Table will be presented.
Why Is DOSY So Different From COSY?
During the 80th session of the Global NMR Discussion Meetings held on February 13th, 2024 via Zoom, Prof. Gareth Morris from the University of Manchester, United Kingdom, gave a talk on the topic "Why is DOSY so different from COSY?". The recording serves as a tutorial.
Abstract:
Peaks in COSY, NOESY, ROESY and HSQC 2D spectra either appear where they should, or not at all. In DOSY spectra, it is all too common to see peaks at incorrect positions in the diffusion domain. This tutorial will explore why this happens, what we can do about it, and how to avoid being misled by DOSY spectra.
Prof. Gareth Morris' research group website and bio: https://nmr.chemistry.manchester.ac.uk/?q=node%2F13
Characterization of polymeric materials in bioapplications
During the 79th session of the Global NMR Discussion Meetings held on January 30th, 2024, via Zoom, Prof. Ann-Christin Pöppler from the University of Würzburg, Germany, gave a talk on the topic "Challenges in the characterization of polymeric materials for drug delivery and tissue engineering applications". The recording serves as a tutorial.
Abstract:
NMR spectroscopy is sensitive to subtle changes in local environment and dynamics over multiple length and time scales making it a versatile technique to study polymeric materials and incorporated molecules. Challenges, which will be discussed in this presentation arise from the size (distribution), rigidity and intrinsic disorder of the samples.
Prof. Ann-Christin Pöppler's research group website: https://www.chemie.uni-wuerzburg.de/oc/poeppler-group/
Single Chip Dynamic Nuclear Polarization Microsystems
During the 78th session of the Global NMR Discussion Meetings held on January 16st, 2024 via Zoom, Dr. Nergiz Sahin Solmaz from EPFL in Lausanne, Switzerland, gave a talk on the topic "Single Chip Dynamic Nuclear Polarization Microsystems". The recording serves as a tutorial.
Abstract:
Dynamic nuclear polarization (DNP) is one of the most powerful and versatile hyperpolarization methods to enhance nuclear magnetic resonance (NMR) signals. A major drawback of DNP is the cost and complexity of the required microwave hardware, especially at high magnetic fields and low temperatures. To overcome this drawback and with the focus on the study of nanoliter and subnanoliter samples, I will present single chip DNP microsystems where the microwave excitation and detection are performed locally on chip without the need of external microwave generators and transmission lines.
Dr. Nergiz Sahin Solmaz's website: https://people.epfl.ch/nergiz.sahin?lang=en
NMR Strategies for Examining Interactions with Nanoparticles
During the 77th session of the Global NMR Discussion Meetings held on December 19st, 2023 via Zoom, Prof. Leah B. Casabianca from Clemson University, United States, gave a talk on the topic "NMR Strategies for Examining Interactions Between Small Molecules and Nanoparticle Surfaces". The recording serves as a tutorial.
Abstract:
Interactions between small molecules and the surface of nanoparticles are important in a variety of fields, from drug delivery to the fate of nanoparticles in the environment. In this talk, I will discuss several NMR techniques that can be used to gain structural and dynamic information about these interactions, with a focus on saturation-transfer difference (STD)-NMR.
Prof. Leah Casabianca's research group website: https://chemistry.sites.clemson.edu/c...
High-Dimensional Spectroscopy to Tackle Complexity in Bio-NMR
During the 75th session of the Global NMR Discussion Meetings held on November 21st, 2023 via Zoom, Prof. Jan Stanek from the University of Warsaw in Poland, gave a talk on the topic "High-dimensional spectroscopy and other tools to tackle complexity in biological solid-state NMR". The recording serves as a tutorial.
Abstract:
Fast (sup. 60 kHz) magic-angle spinning provides high sensitivity and narrowed 1H linewidths. However, spectral analysis of large proteins are often severely hampered by peak overlap and/or ambiguity, depending on system size and sample quality. In this tutorial lecture, I will discuss several experimental techniques to address this, namely high-dimensional (4D, 5D) spectroscopy with non-uniform sampling, projection spectroscopy and time-shared acquisition. Additionally, I will explore selected tools for automation of resonance assignment and present early data on protein dynamics measurement as pseudo-4D series.
Website: http://nmr.cent3.uw.edu.pl/stanek
Scaling Analyses of Hyperpolarization Transfer in Solids
During the 74th session of the Global NMR Discussion Meetings held on November 7th, 2023 via Zoom, Prof. Bradley F. Chmelka from the University of California in the U.S., gave a talk on the topic "Scaling Analyses of Hyperpolarization Transfer in Solids and across Interfaces". The recording serves as a tutorial.
Abstract: Classical scaling analyses, with analogies to heat conduction and mass transfer, quantitatively describe the propagation and dissipation of non-Boltzmann spin polarization in heterogeneous solids. The analyses yield general design criteria for predicting, analyzing, and optimizing polarization transfer within solids and across interfaces between dissimilar materials.
Inside an NMR Spectrometer
During the 73rd session of the Global NMR Discussion Meetings held on October 24th, 2023 via Zoom, Prof. Kazuyuki Takeda from Kyoto University in Japan, gave a talk on the topic "Inside an NMR Spectrometer". The recording serves as a tutorial.
Abstract: Let us take a brief look at what is happening inside an NMR spectrometer when we operate it, running pulse sequences and acquiring NMR signals. In particular, I will focus on open-resource, home-built NMR spectrometers we routinely use in our lab, and show how we apply them in the conventional and unconventional NMR experiments.
Website: http://kuchem.kyoto-u.ac.jp/bun/indiv/takezo/index_en.html http://www.kuchem.kyoto-u.ac.jp/organization/member/bk_200710/takezo_e.html
Explorations in NMR Relaxation
During the 72nd session of the Global NMR Discussion Meetings held on October 10th, 2023 via Zoom, Prof. Alexej Jerschow gave a talk on the topic "Explorations in NMR Relaxation by Experiments and MD/Ab Initio Computation". The recording serves as a tutorial.
Prof. Alexej Jerschow is a Professor of Chemistry at New York University, USA.
Website: https://wp.nyu.edu/jerschow/
Google scholar: https://scholar.google.com/citations?...
X (previously Twitter): https://twitter.com/ajerschow
NMR Pulse Sequence Basics and Design Principles
During the 71st session of the Global NMR Discussion Meetings held on September 26th, 2023 via Zoom, Prof. Tairan Yuwen gave a talk on the topic "NMR pulse sequence basics and design principles". The recording serves as a tutorial.
Prof. Tairan Yuwen is an Assistant Professor at Peking University, China
Abstract: NMR is commonly used for studying structure and dynamics of molecules, and many NMR experiments have been developed for different purposes. In each NMR experiment it is necessary to choose the most suitable NMR pulse sequence to obtain optimal results, which requires understanding about the basic theory. During NMR pulse sequence the studied system is evolved under different types of interactions, which can be represented by density matrix or product operator. The basic syntax for NMR pulse sequence programming will be briefly introduced together with several examples, which helps to understand how NMR pulse sequences work and make further optimization.