Videos
Integrative Structural Biology: Challenges and Opportunities for MR
During the 93rd session of the Global NMR Discussion Meetings held on December 3rd, 2024, via Zoom, Prof. Tatyana Polenova from the University of Delaware, USA, gave a talk on the topic "Integrative Structural Biology of Protein Assemblies: Challenges and Opportunities for Magnetic Resonance". The recording serves as a tutorial.
Abstract: I will present recent developments in MAS NMR techniques for atomic-resolution structural analysis of large biological assemblies. Drawing on studies of diverse systems from our lab, including the kinesin motor domain with polymerized microtubules, cofilin bound to filamentous actin, and HIV-1 protein assemblies interacting with small-molecule maturation inhibitors, I will illustrate how with MAS NMR, structural and dynamic details, as well as drug interactions were uncovered, that are inaccessible using other approaches. Additionally, I will discuss the benefits of combining MAS NMR with medium-resolution cryo-EM and MD simulations to enhance structural understanding, and address the current challenges and future directions for magnetic resonance in integrative structural biology.
Find out more about Prof. Tatyana Polenova's research: https://www.udel.edu/faculty-staff/experts/tatyana-polenova/
NMR approaches for intrinsically disordered proteins
During the 91st session of the Global NMR Discussion Meetings held on November 5th, 2024, via Zoom, Prof. Julie Forman-Kay from the University of Toronto & SickKids Research Institute, Canada, gave a talk on the topic "NMR approaches for intrinsically disordered proteins". The recording serves as a tutorial.
Abstract: NMR is a powerful tool for obtaining site-specific information about dynamic systems, including intrinsically disordered proteins and protein regions (IDPs/IDRs) which do not adopt unique, stable folded structures. While computational approaches are increasingly powerful for stable proteins and protein domains, there is a huge need for experimental information about IDPs, IDRs and the highly dynamic complexes that they often make with other disordered proteins, folded domains and other biomolecules. The lecture will highlight examples of NMR studies of IDPs and their dynamic complexes, including condensed state models of those found in biomolecular condensates.
Find out more about Prof. Julie Forman-Kay's research: https://biochemistry.utoronto.ca/person/julie-d-forman-kay/
Hyperpolarized Xe NMR in Molecular Cavities Study and MR Imaging
During the 83rd session of the Global NMR Discussion Meetings held on March 26th, 2024 via Zoom, Prof. Leif Schröder from the German Cancer Research Center gave a talk on the topic "Hyperpolarized Xenon NMR for Exploring Molecular Host Cavities and Advancing MR Imaging". The recording serves as a tutorial.
Abstract: The large chemical shift range of Xe-129 NMR makes this nucleus a valuable probe in spectroscopy and biomedical imaging. Hyperpolarized Xe can be used also as dissolved noble gas to provide 10’000-fold improved sensitivity over extended periods of time. In combination with saturation transfer techniques, reversibly bound hyperpolarized xenon gives insights into exchange kinetics of various host-guest complexes. This talk will give an overview of studies investigating the affinity of dissolved Xe for various hosts like biogenic hollow protein structures with attoliter volumes or synthetic hosts for trapping individual Xe atoms. Both strategies find applications in the spectroscopic investigation of exchange kinetics of host-guest systems and in the design of ultra-sensitive magnetic resonance imaging agents.
Prof. Schroeder's research: https://www.dkfz.de/en/translationale-molekulare-bildgebung/index.php
NMR Metabolomics in Drug Discovery and Disease Diagnosis
During the 82nd session of the Global NMR Discussion Meetings held on March 12th, 2024 via Zoom, Prof. Robert Powers from the University of Nebraska-Lincoln, United States, gave a talk on the topic "The Application of NMR Metabolomics to Drug Discovery and Disease Diagnosis". The recording serves as a tutorial.
Abstract: NMR-based metabolomics has benefited a variety of fields including drug discovery and disease diagnosis. Metabolomics is technically very challenging and requires expertise in a diversity of scientific areas. In this regard, this tutorial will discuss best practices for sample preparation and handling, data collection and analysis, and statistical modeling and interpretation to achieve biologically and clinically significant results.
Prof. Robert Powers' research group website and bio: https://chem.unl.edu/robert-powers
Characterization of polymeric materials in bioapplications
During the 79th session of the Global NMR Discussion Meetings held on January 30th, 2024, via Zoom, Prof. Ann-Christin Pöppler from the University of Würzburg, Germany, gave a talk on the topic "Challenges in the characterization of polymeric materials for drug delivery and tissue engineering applications". The recording serves as a tutorial.
Abstract:
NMR spectroscopy is sensitive to subtle changes in local environment and dynamics over multiple length and time scales making it a versatile technique to study polymeric materials and incorporated molecules. Challenges, which will be discussed in this presentation arise from the size (distribution), rigidity and intrinsic disorder of the samples.
Prof. Ann-Christin Pöppler's research group website: https://www.chemie.uni-wuerzburg.de/oc/poeppler-group/
NMR Strategies for Examining Interactions with Nanoparticles
During the 77th session of the Global NMR Discussion Meetings held on December 19st, 2023 via Zoom, Prof. Leah B. Casabianca from Clemson University, United States, gave a talk on the topic "NMR Strategies for Examining Interactions Between Small Molecules and Nanoparticle Surfaces". The recording serves as a tutorial.
Abstract:
Interactions between small molecules and the surface of nanoparticles are important in a variety of fields, from drug delivery to the fate of nanoparticles in the environment. In this talk, I will discuss several NMR techniques that can be used to gain structural and dynamic information about these interactions, with a focus on saturation-transfer difference (STD)-NMR.
Prof. Leah Casabianca's research group website: https://chemistry.sites.clemson.edu/c...
High-Dimensional Spectroscopy to Tackle Complexity in Bio-NMR
During the 75th session of the Global NMR Discussion Meetings held on November 21st, 2023 via Zoom, Prof. Jan Stanek from the University of Warsaw in Poland, gave a talk on the topic "High-dimensional spectroscopy and other tools to tackle complexity in biological solid-state NMR". The recording serves as a tutorial.
Abstract:
Fast (sup. 60 kHz) magic-angle spinning provides high sensitivity and narrowed 1H linewidths. However, spectral analysis of large proteins are often severely hampered by peak overlap and/or ambiguity, depending on system size and sample quality. In this tutorial lecture, I will discuss several experimental techniques to address this, namely high-dimensional (4D, 5D) spectroscopy with non-uniform sampling, projection spectroscopy and time-shared acquisition. Additionally, I will explore selected tools for automation of resonance assignment and present early data on protein dynamics measurement as pseudo-4D series.
Website: http://nmr.cent3.uw.edu.pl/stanek
Dihedral Angle Measurements by Solid-State NMR
During the 70th session of the Global NMR Discussion Meetings held on August 29th, 2023 via Zoom, Prof. Patrick van der Wel gave a talk on the topic "Dihedral angle measurements by solid-state NMR". The recording serves as a tutorial.
Prof. Patrick van der Wel is an Associate Professor at the University of Groningen, Netherlands.
Abstract: Dihedral angle measurements represent an important complement to distance-based structural data in solid-state NMR studies of (bio)molecular structure. In this tutorial I will discuss the role of angular restraints in ssNMR structure analysis, historical and recent case studies, and the underlying principles of representative approaches.
Website: https://vanderwellab.org/
Google scholar: https://scholar.google.com/citations?...
Twitter: https://twitter.com/p_vanderwel
Magnetic Resonance Spectroscopy Using Spin Defects in Diamond
During the 69th session of the Global NMR Discussion Meetings held on June 13th, 2023 via Zoom, Prof. Dominik Bucher gave a talk on the topic "Nano- and Microscale Magnetic Resonance Spectroscopy Using Spin Defects in Diamond". The recording serves as a tutorial.
Abstract: Nuclear magnetic resonance (NMR), one of the most powerful analytical techniques in chemistry and life sciences, is typically limited to macroscopic volumes due to its inherent low sensitivity. This excludes NMR spectroscopy from the analysis of microscopic sample sizes, such as in single-cell biology or microfluidic applications. In recent years, it has been shown that NMR signals from nano- to microscale volumes can be detected by a new class of sensors - quantum sensors based on defects in the diamond lattice - the nitrogen vacancy (NV) centre. In this talk, I will first introduce NV centres and explain how these atom-sized sensors can be used to detect NMR signals. In the second part, I will provide an overview of this rapidly developing technology and discuss potential applications ranging from surface and materials science to lab-on-a-chip applications.
Prof. Dominik Bucher is a Rudolf-Mößbauer Professor at the Technical University of Munich (TUM), Germany.
Website: https://www.ch.nat.tum.de/en/qsens/home/
Google scholar: https://scholar.google.de/citations?u...
Twitter: https://twitter.com/Bucherlab
Measuring Dynamics Using Anisotropic Interactions in MAS-NMR
During the 68th session of the Global NMR Discussion Meetings held on May 30th, 2023 via Zoom, Dr. Kaustubh Mote gave a talk on the topic "Measuring dynamics using anisotropic interactions in MAS-NMR". The recording serves as a tutorial.
Abstract: Solid-state MAS NMR gives direct access to anisotropic interactions such as CSA, dipole-dipole and quadrupolar couplings. The precise measurement of these interactions, in addition to providing structural features, also gives access to dynamics via their partial averaging due to molecular motion. This tutorial will explore various techniques that are available to extract details of molecular motion via the measurement of these anisotropic interactions, with a particular focus on the measurement of dipole-dipole couplings.
Dr. Kaustubh Mote is a Reader at Tata Institute of Fundamental Research (TIFR) Hyderabad, India.
Website: https://www.tifrh.res.in/~kaustubh/
Google scholar: https://scholar.google.com/citations?...
Twitter: https://twitter.com/kaustubhmote_?lan...
NMR Crystallography: Integrative Foundations and Applications
During the 64th session of the Global NMR Discussion Meetings held on March 21st, 2023 via Zoom, Prof. Leonard Mueller gave a talk on the topic "NMR Crystallography: Integrative Foundations and Applications to Materials Chemistry and Structural Biology". The recording serves as a tutorial.
Abstract: NMR crystallography – the integrated application of solid-state NMR, X-ray diffraction, and first-principles computational chemistry – is rapidly developing as an atomic-resolution probe of structure and function across the molecular sciences. Here, I will discuss critical aspects in the integration of these three techniques, including the need for a priori determination of linear rescaling parameters and rigorous methods of statistical analysis. I will present two recent examples from my group's work that highlight these factors. The first is to photomechanical materials, connecting the molecular-level structural rearrangement to the experimentally-observed macroscopic response. The second is to integrative structural biology, revealing chemically-detailed structure and dynamics in the enzyme active site of tryptophan synthase, and how this has changed our understanding of its mechanism and inhibition.
Prof. Leonard Mueller is a Professor of Chemistry at the University of California Riverside, USA.
Website: https://sites.google.com/ucr.edu/muel...
Google scholar: https://scholar.google.com/citations?...
Chemical Exchange Saturation Transfer (CEST) MRI
During the 62nd session of the Global NMR Discussion Meetings held on February 21st, 2023 via Zoom, Prof. Elena Vinogradov gave a talk on the topic "Chemical Exchange Saturation Transfer (CEST) MRI: from basic principles to potential clinical applications". The recording serves as a tutorial.
Abstract:
Novel MRI methods are focusing on imaging events and structures at the molecular level. One of the methods gaining popularity is Chemical Exchange Saturation Transfer (CEST). Here we will discuss basic physical principles of this approach, followed by potential applications and challenges associated with the translation to in-vivo and human imaging.
Prof. Elena Vinogradov is an associate Professor at UT Southwestern Medical Center in Dallas, Texas.
Microfluidics & Magnetic Resonance: A multidisciplinary challenge
During the 60th session of the Global NMR Discussion Meetings held on January 24th, 2023 via Zoom, Prof. Marcel Utz gave a talk on the topic "Microfluidics & Magnetic Resonance: A multidisciplinary challenge". The recording serves as a tutorial.
Abstract:
Microfluidic technology has rapidly advanced over the last two decades, and is increasingly transforming the practice of life science research, as well as (arguably more slowly) medical diagnostics. In particular, microfluidic assays are ideal platforms for the culture of cells, cell aggregates, and tissue slices, and form the basis of increasingly predictive disease models. Nuclear magnetic resonance, due to its non-invasive nature and ability to provide rich and detailed information on biological systems, is ideally suited to follow life processes in such microfluidic culture devices. However, the integration of high-performance NMR spectroscopy with microfluidic lab-on-a-chip devices is technically challenging due to the small sample volumes involved. Recent advances in hyperpolarization and alternative detection approaches offer important opportunities in this field. In this talk, I will give an overview of the opportunities and challenges in microfluidic NMR, and present some recent examples.
Prof. Marcel Utz is Professor of Magnetic Resonance, Microfluidics, and Complex Materials within Chemistry at the University of Southampton.
Website: https://utzgroup.ddns.net
Google scholar: https://scholar.google.com/citations?...
EPR-NMR for Protein Modeling
During the 57th session of the Global NMR Discussion Meetings held on November 29th, 2022 via Zoom, Prof. Gunnar Jeschke from ETH Zurich, gave a talk on the topic "Integrating EPR distance distribution restraints with NMR data in protein models". The recording serves as a tutorial.
Abstract:
This zoominar will start with a short explanation of in silico spin labelling, which is useful for planning paramagnetic relaxation enhancement (PRE) NMR experiments as well as DEER EPR experiments for measuring distance distributions. I will continue with a primer on converting DEER data into distance distributions. The zoominar concludes with an example of using distance distributions in modelling a weakly structured low-complexity domain of an RNA-binding protein.
Speaker's biography:
1993-1996 PhD at ETH Zurich, Switzerland, with A. Schweiger
1998-2006 Group Leader, Max Planck Institute for Polymer Science in Mainz, Germany, with H. W. Spiess
2006-2008 Professor at the University of Konstanz, Germany
2008- Professor at ETH Zurich
Follow Prof. Jeschke's work here:
Website: https://epr.ethz.ch/
Using Deep Learning to Transform and Analyse NMR Data
During the 55th session of the Global NMR Discussion Meetings held on November 1st, 2022 via Zoom, Prof. Flemming Hansen from University College London, gave a talk on the topic "Using Deep Learning to Transform and Analyse NMR Data". The recording serves as a tutorial.
It will be shown how deep neural networks (DNNs) can be trained for reconstruction of sparsely sampled NMR spectra and for virtual homonuclear decoupling. Another area covered in the talk will be autonomous analysis of chemical exchange saturation transfer (CEST) data, where the trained DNN accurately predicts both the chemical shifts and the uncertainties associated with these.
Speaker's biography:
1997-2003: BSc+MSc, Chemistry + Physics, University of Copenhagen
2003-2005: PhD in Biophysical Chemistry (Prof. Jens Led) , University of Copenhagen
2005-2010: Postdoctoral fellow (Prof Lewis E. Kay), University of Toronto
2010-present: PI, University College London. Full Professor since 2017.
Follow Prof. Hansen's work here:
Twitter: https://twitter.com/dflemminghansen?l...
Google scholar: https://scholar.google.com/citations?...
Website: https://www.ucl.ac.uk/hansen-lab/flem...
Emerging frontiers in solution NMR of large protein systems
During the 54th session of the Global NMR Discussion Meetings held on October 18th, 2022 via Zoom, Prof. Haribabu Arthanari from Harvard Medical School, gave a talk on the topic "Emerging frontiers to address large protein systems by solution NMR". The recording serves as a tutorial.
Speaker's biography:
1994-1996: MSc Chemistry - IIT Madras, India
1996-2003: PhD - Wesleyan University, USA
2003-2010: Research Associate - Harvard Medical School, USA
2010-2016: Lecturer - Harvard Medical School, USA
2016-present: Professor - Harvard Medical School, USA
Follow Prof. Arthanari's work here:
Google scholar: https://scholar.google.com/citations?...
Website: https://artlab.dana-farber.org/
Parahydrogen-based Exchange Chemistry for Next-Generation MR
The Fall 2022 series of meetings began on September 20th with a talk by Dr. Danila Barskiy on the topic "Parahydrogen-based Exchange Chemistry for Next-Generation Magnetic Resonance". The recording serves as a tutorial.
Abstract:
In my talk, I will present our recent work demonstrating that parahydrogen-based spin chemistry can generate hyperpolarized molecules (urea, alcohols, amino acids, ammonium, glucose, etc.) for benchtop (1 tesla) NMR as well as for zero- to ultralow-field (ZULF) NMR. I will describe the basics of exchange-based hyperpolarization and discuss potential applications for chemical analysis.
Speaker's biography:
Dr. Danila A. Barskiy
PhD - Novosibirsk University, 2012 - 2015
Postdoc - Vanderbilt University, 2015 - 2017
Postdoc - University of California Berkeley, 2017 - 2020
Presently, he is a research Group Leader at the Helmholtz Institute, Johannes Gutenberg University Mainz. His group works on chemistry-oriented hyperpolarization technologies for affordable nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI).
Follow Dr. Barskiy's work here:
Google scholar: https://scholar.google.com/citations?...
Website: https://budker.uni-mainz.de/?page_id=70
Twitter: https://twitter.com/tovarishbarskiy
1H Detection, MAS & High Fields for Membrane Proteins
During the 49th session of the Global NMR Discussion Meetings held on June 14, 2022 via Zoom, Dr. Loren Andreas from Max Planck Institute for Multidisciplinary Sciences gave a talk on the topic "Proton Detection, Magic-Angle Spinning and High Magnetic Fields for Membrane Protein Structural ". The recording serves as a tutorial.
Abstract:
I will present several topics, starting with the practical aspects of setting up proton-detection measurements with 1.3 mm and 0.7 mm rotors capable of 60 and greater than 100 kHz spinning. From there, I will highlight some of our recent results including detection of bound water, hydrogen bonding interaction, as well as new developments in pulse sequences aimed at global structure determination. I may also briefly mention some DNP results.
Speaker's Biography:
2016-Present: Professor, Group Leader, MPI Göttingen, Germany
2014-2016: Postdoc ENS, Lyon, France
2014: Ph.D. MIT, Boston, USA
Website: https://www.mpinat.mpg.de/andreas
Google scholar: https://scholar.google.com/citations?...
Structure and Dynamics of Viruses by MAS NMR
During the 48th session of the Global NMR Discussion Meetings held on May 17, 2022 via Zoom, Gal Porat-Dahlerbruch from the University of Delaware gave a talk on the topic "Structure and Dynamics of Viruses by MAS NMR". The recording serves as a tutorial.
Abstract:
Magic angle spinning (MAS) NMR has been widely used to characterize the structure and dynamics of viruses at all levels of organization. In this talk, I will cover the basics of MAS NMR spectroscopy with specific emphasis on experiments designed to study viruses, discuss the advantages and complementarity of MAS NMR with respect to other structural biology techniques, and present examples of unique insights gained into HIV-1, Influenza A, Hepatitis B, and other viruses.
Read their recent review here:
Gal Porat-Dahlerbruch, Amir Goldbourt, Tatyana Polenova (2021) Virus Structures and Dynamics by Magic-Angle-Spinning NMR. Annual Review in Virology. 8(1):219-237. DOI: 10.1146/annurev-virology-011921-064653.
Speaker's biography:
2015-2018: BS Chemistry, Tel Aviv University, Israel
2018-2019: Graduate Research Assistant, Tel Aviv University, Israel (Prof. Amir Goldbourt)
2019-Present: Ph.D. Candidate, University of Delaware, USA (Prof. Tatyana Polenova)
Twitter: @MagicAngleGal
Polenova laboratory website: https://sites.udel.edu/polenova-group/
Solid-State NMR and DNP of Cellular Carbohydrates
During the 43rd session of the Global NMR Discussion Meetings held on February 22nd, 2022 via Zoom, Prof. Tuo Wang gave a talk on the topic "Cellular Carbohydrates: Limitations and Opportunities from Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP)". The recording serves as a tutorial.
Abstract: Solid-State NMR has demonstrated its unique capability of characterizing the highly polymorphic structure of carbohydrate polymers in living cells or intact tissues. Here we will present the recent applications to plant, fungi, and microalgal systems and discuss the new opportunities enabled by ultrahigh-field magnets and DNP.
Website: https://www.chemistry.msu.edu/faculty-research/faculty-members/wang-tuo.aspx
Google Scholar: https://scholar.google.com/citations?...