Time-resolved solid-state NMR: Overview, Application in Biophysics
During the 38th session of the Global NMR Discussion Meetings on Zoom, Dr. Blake Wilson, National Institute of Health (NIH) Bethesda, gave a tutorial on "Time-resolved solid-state nuclear magnetic resonance (NMR) spectroscopy: an overview, with applications to biophysical systems".
Bio of Dr. Blake Wilson:
2013: B.S. Physics, Massachusetts Institute of Technology (Prob. Bob Griffin)
2013 - 2019: PhD, University of California, Santa Barbara (Prof. Mark Sherwin and Prof. Songi Han)
2019 - Present: Postdoctoral fellow, NIH Bethesda (Dr. Rob Tycko)
Follow Dr. Wilson and his work:
Twitter: https://twitter.com/blkwiln
Google scholar: https://scholar.google.com/citations?...
Abstract: I will give an overview of time-resolved solid-state NMR techniques, and how they can be used to study structural conversion processes in biophysical systems. A variety of methods, including rapid pH jumps, rapid temperature jumps, and rapid mixing of different species, can be used to initiate biophysical processes, which can subsequently be probed with millisecond time resolution by sudden freezing followed by interrogation with solid-state NMR. I will discuss how dynamic nuclear polarization (DNP) has been shown to dramatically improve the sensitivity of time-resolved NMR techniques, with the aim of capturing structural information from sparsely populated intermediate states.