Videos
Understanding DNP : Insights from Average Hamiltonian Theory
During the 90th session of the Global NMR Discussion Meetings held on October 22nd, 2024 via Zoom, Prof. Sheetal Kumar Jain from the Indian Institute of Science, Bengaluru, India, gave a talk on the topic "Understanding Dynamic Nuclear Polarization: Insights from Average Hamiltonian Theory". The recording serves as a tutorial.
Abstract: Average Hamiltonian Theory (AHT) effectively explains the mechanisms, efficiencies, and optimal conditions for solid-state NMR pulse sequences. In Dynamic Nuclear Polarization (DNP), AHT describes how electron-nuclear spin couplings drive polarization transfer under microwave irradiation. By simplifying spin dynamics, AHT offers insights into polarization mechanisms like the solid effect, cross effect, and pulsed DNP, helping predict conditions to maximize nuclear spin polarization and enhance NMR signal sensitivity.
Find out more about Prof. Sheetal Kumar Jain's research: https://sscu.iisc.ac.in/sheetal-kumar-jain/
Electron Paramagnetic Resonance Advancing MAS-DNP
During the 88th session of the Global NMR Discussion Meetings held on October 1st, 2024 via Zoom, Dr. Frédéric Mentink-Vigier from the National High Magnetic Field Laboratory, Florida, US gave a talk on the topic "Electron Paramagnetic Resonance Advancing Magic Angle Spinning Dynamic Nuclear polarization". The recording serves as a tutorial.
Abstract: Electron Paramagnetic Resonance (EPR) is crucial for analyzing paramagnetic species used in Dynamic Nuclear Polarization (DNP). This overview covers EPR fundamentals and its application to studying biradicals, which are key for high-field Magic Angle Spinning (MAS) DNP.
Find out more about Dr. Frédéric Mentink-Vigier's work: https://nationalmaglab.org/staff/?name=FredericMentink-vigier
Single Chip Dynamic Nuclear Polarization Microsystems
During the 78th session of the Global NMR Discussion Meetings held on January 16st, 2024 via Zoom, Dr. Nergiz Sahin Solmaz from EPFL in Lausanne, Switzerland, gave a talk on the topic "Single Chip Dynamic Nuclear Polarization Microsystems". The recording serves as a tutorial.
Abstract:
Dynamic nuclear polarization (DNP) is one of the most powerful and versatile hyperpolarization methods to enhance nuclear magnetic resonance (NMR) signals. A major drawback of DNP is the cost and complexity of the required microwave hardware, especially at high magnetic fields and low temperatures. To overcome this drawback and with the focus on the study of nanoliter and subnanoliter samples, I will present single chip DNP microsystems where the microwave excitation and detection are performed locally on chip without the need of external microwave generators and transmission lines.
Dr. Nergiz Sahin Solmaz's website: https://people.epfl.ch/nergiz.sahin?lang=en
Scaling Analyses of Hyperpolarization Transfer in Solids
During the 74th session of the Global NMR Discussion Meetings held on November 7th, 2023 via Zoom, Prof. Bradley F. Chmelka from the University of California in the U.S., gave a talk on the topic "Scaling Analyses of Hyperpolarization Transfer in Solids and across Interfaces". The recording serves as a tutorial.
Abstract: Classical scaling analyses, with analogies to heat conduction and mass transfer, quantitatively describe the propagation and dissipation of non-Boltzmann spin polarization in heterogeneous solids. The analyses yield general design criteria for predicting, analyzing, and optimizing polarization transfer within solids and across interfaces between dissimilar materials.
Triplet Dynamic Nuclear Polarization
During the 66th session of the Global NMR Discussion Meetings held on May 2nd, 2023 via Zoom, Prof. Nobuhiro Yanai gave a talk on the topic "Triplet Dynamic Nuclear Polarization: New Polarizing Agents and Targets". The recording serves as a tutorial.
Abstract:
Triplet dynamic nuclear polarization (triplet-DNP), which utilizes the polarized electron spins of the photo-excited triplet, is an excellent method to obtain large nuclear spin polarization even under mild conditions below 1 T near room temperature. This talk will highlight the potential of triplet-DNP and the exciting new possibilities that have emerged with recent advancements in polarizing agents and targets. Specifically, I will cover the following topics:
- Mechanism of triplet-DNP as an introduction
- Expanding the targets using supramolecular polarizing agents
- Improving air stability and solubility with non-pentacene polarizing agents
- Challenging polarization transfer with the use of nanomaterials.
Nobuhiro Yanai is an Associate Professor, Department of Chemistry and Biochemistry, Kyushu University, Japan.
Website: https://sites.google.com/view/nobuhiroyanai/
Google scholar: https://scholar.google.com/citations?...
Twitter: https://twitter.com/nobuhiroyanai?lang=en
Solid-State NMR for Investigating Crystallization from Solution
During the 61st session of the Global NMR Discussion Meetings held on February 7th, 2023 via Zoom, Dr. Giulia Mollica gave a talk on the topic "Solid-state NMR for investigating crystallization from solution". The recording serves as a tutorial.
Abstract:
Crystallization underpins essential processes in our everyday life, creating exceptional materials. Yet, fundamental understanding of the mechanisms underlying crystallization processes is still lacking because of the scarcity of experimental approaches allowing atomic-level investigation of the sequence of intermediate phases formed during crystallization as a function of time. After introducing the basic principles of crystallization and the associated experimental challenges and current analytical approaches, I will discuss how NMR, coupled with hyperpolarization methods, can contribute to solve long standing questions on crystallization, with particular attention to organic polymorphic compounds. Current limitations and possible future directions will be discussed.
Dr. Giulia Mollica is a Research Director at the CNRS at the Institut de Chimie Radicalaire in Marseille, France.
Website: https://mollicalab.fr/
Google scholar: https://scholar.google.co.uk/citation...
twitter: https://twitter.com/giuliamollica
Surprising Consequences of High Electron Spin Polarization
During the 59th session of the Global NMR Discussion Meetings held on January 10th, 2023 via Zoom, Dr. Quentin Chappuis Stern gave a talk on the topic "The surprising consequences of near unity electron spin polarization". The recording serves as a tutorial.
Abstract:
The inherent low sensitivity of NMR can be overcome by hyperpolarization techniques, which increase the polarization of nuclear spins far beyond the Boltzmann equilibrium. One such method is dynamic nuclear polarization (DNP), which consists of transferring the high polarization of unpaired electron spins to surrounding nuclear spins via microwave irradiation. In the case of dissolution DNP (dDNP), the sample is hyperpolarized in the solid state at low temperature (1-2 K) and moderate magnetic field (3-7 T), where the Boltzmann polarization of electron spins approaches unity, translating into equally high nuclear polarization under DNP. In addition to yielding high nuclear polarization, the high electron polarization has other interesting consequences for NMR. Indeed, as the electron polarization tends towards unity, the electron flip-flop probability vanishes, an effect sometimes referred to as “bath quenching”. By switching on and off microwave irradiation (microwave gating), one switches on and off electron flip-flops and hence paramagnetic relaxation. This has been shown to have a dramatic effect on transverse nuclear relaxation and on nuclear spin diffusion. In this talk, I will review the basic concepts of paramagnetic relaxation and its dependence on electron polarization. I will then present experiments where we used microwave gating to perform efficient CP, to detect EPR properties indirectly via NMR properties, and finally to study nuclear spin diffusion in the vicinity of electron spins.
Speaker's biography:
2014- Undergraduate and Masters, EPFL (Switzerland). Thesis with Prof. Bodenhausen
2022- PhD, CRMN Lyon (France), with Prof. Sami Jannin
Follow Dr. Stern's work here:
Twitter: https://twitter.com/quentinchapp
Google scholar: https://scholar.google.fr/citations?u...
Room Temperature DNP via P1 Centers in Diamond
During the 58th session of the Global NMR Discussion Meetings held on December 14th, 2022 via Zoom, Prof. Chandrasekhar Ramanathan from Dartmouth College, USA , gave a talk on the topic "Room Temperature DNP via substitutional-nitrogen (P1) centers in diamond". The recording serves as a tutorial.
Abstract:
Electron spins in diamond have long coherence and relaxation times at room temperature, making them an exciting platform for EPR and DNP experiments under ambient conditions. In this talk I will describe our recent W-band DNP experiments using the substitutional nitrogen (or P1) defect to hyperpolarize the 13C spins in both single crystal and diamond powders. The DNP spectra measured in these samples show signatures of multiple mechanisms including the solid effect, the cross effect, the truncated cross effect and the Overhauser effect. I will discuss how variations in the microscopic environments of the spins could lead to these observations.
Follow Prof. Ramanathan's work here:
Twitter: https://twitter.com/sekharspins
Google scholar: https://scholar.google.com/citations?...
Website: https://sites.dartmouth.edu/quantum-s...
Paramagnetic effects in NMR: From PRE to metal ions DNP
During the 52nd session of the Global NMR Discussion Meetings held on October 4th, 2022 via Zoom, Dr. Daniel Jardon Alvarez from Weizmann Institute of Science gave a talk on the topic "Paramagnetic effects in NMR: From PRE to metal ions based DNP ". The recording serves as a tutorial.
Abstract:
DNP enhancements are distance independent as long as the nuclear relaxation is governed by paramagnetic relaxation enhancement (PRE) from the polarizing agents. This enables large and homogeneous enhancements in the bulk of inorganic materials upon doping with paramagnetic metal ions. In this talk I will present the basics of PRE and solid effect DNP and, from there, justify the initial statement.
Speaker's biography:
2006-2012: BSc, MSc - Ludwig-Maximilians University, Germany
2012-2016: PhD - University of São Paulo, Brazil
2016-2019: Postdoc - Ohio State University, USA
2019-2021: Postdoc - Weizmann Institute, Israel
2021-present: Senior Intern - Weizmann Institute, Israel
Follow Dr. Jardon Alvarez's work here:
Google scholar: https://scholar.google.com/citations?...
Twitter: @dani_jardon
Review on DNP from metal ion dopants: https://www.sciencedirect.com/science...
Solid-State NMR and DNP of Cellular Carbohydrates
During the 43rd session of the Global NMR Discussion Meetings held on February 22nd, 2022 via Zoom, Prof. Tuo Wang gave a talk on the topic "Cellular Carbohydrates: Limitations and Opportunities from Solid-State Nuclear Magnetic Resonance (NMR) and Dynamic Nuclear Polarization (DNP)". The recording serves as a tutorial.
Abstract: Solid-State NMR has demonstrated its unique capability of characterizing the highly polymorphic structure of carbohydrate polymers in living cells or intact tissues. Here we will present the recent applications to plant, fungi, and microalgal systems and discuss the new opportunities enabled by ultrahigh-field magnets and DNP.
Website: https://www.chemistry.msu.edu/faculty-research/faculty-members/wang-tuo.aspx
Google Scholar: https://scholar.google.com/citations?...
Long-lived states: some unexpected applications
During the 42nd session of the Global NMR Discussion Meetings held on February 8th, 2022 via Zoom, Prof. Geoffrey Bodenhausen gave a talk on the topic "Long-lived states: some unexpected applications". The recording serves as a tutorial.
Abstract: The invention of singlet states, also known as long-lived states (LLS), by Malcolm Levitt was to be followed by many further inventions by scores of creative co-workers in Southampton and Novosibirsk: the quest for molecules immersed in environments capable of supporting singlet states with very long lifetimes, magnetization-to-singlet conversion (M2S), extremely low frequency (ELF) excitation, classification of decay rates according to the eigenvalues of the Liouvillian, effects of exchange on the lifetimes of LLS, etc. Our laboratories at EPFL and ENS have focused on a few applications of long-lived states, such as the study of slow diffusion of macromolecules, long-lived states in substrates of enzymatic reactions, long-lived states in systems with many spins, long-lived population imbalances in systems containing quadrupolar nuclei, elusive long-lived states in water (“para-water”), and long-lived states in drug molecules binding to macromolecular targets.
Speaker's biography:
1996-present: Professor, Department of Chemistry, Ecole Normale Supérieure, Paris
2001-2016: Professor, Ecole Polytechnique Fédérale de Lausanne
1985-2001: Université de Lausanne
1994-1996: National High Magnetic Field Laboratory and Florida State University, Tallahassee
1980-1985: Post-doc at ETH with Richard Ernst
1977 :D. Phil. Oxford with Ray Freeman Corresponding member of the Netherlands Academy of Sciences (KNAW), Fellow of the American Physical Society, Latsis Prize of the Swiss National Science Foundation
Follow Prof. Bodenhausen's work here:
Google scholar: https://scholar.google.fr/citations?u...
Overhauser Dynamic Nuclear Polarization in the Liquid State
During the 40th session of the Global NMR Discussion Meetings held on December 7th, 2021 via Zoom, Marcel Levien, Ph.D. student in Prof. Marina Bennati's laboratory at Max Planck, Göttingen, Germany, gave a talk on "Overhauser Dynamic Nuclear Polarization in the liquid state at room temperature at low and high magnetic fields". The recording serves as a tutorial.
Abstract: Dynamic Nuclear Polarization (DNP) is a tool that promises to overcome the long-standing sensitivity issues in modern Nuclear Magnetic Resonance (NMR). While it is already commercially available in the solid-state, in the liquid state at room temperature the development at high magnetic field is still in its infancy. However, recent advances in the understanding of the polarization transfer mechanisms and in the hardware technology demonstrate the viability of DNP in liquids at high magnetic fields. During this talk, the general Overhauser mechanism for the liquid state will be discussed. Particularly, the polarization transfer via scalar interaction, its frequency dependence as well as the role of the polarizing agent will be explained. Finally, an overview about recent applications at low field as well as developments at high magnetic field will be given.
Speaker's biography:
2012 – 2015: BSc. Chemistry, University of Göttingen, Germany
2015 – 2017: MSc. Chemistry, University of Göttingen, Germany
2018 – present: PhD student, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Practicalities of SPINACH - a tutorial lecture
In Session 33 of the Global NMR Discussion Meetings, Prof. Ilya Kuprov gave a second talk on performing nuclear magnetic resonance (NMR) simulations using the SPINACH program, with an emphasis on practical aspects.
Watch Part 1 here: https://youtu.be/3fnRgGCKnLU
Download related resources from Prof. Kuprov: https://www.dropbox.com/sh/55kn4enfve...
Website and SPINACH download: https://spindynamics.org
Google Scholar: https://scholar.google.com/citations?...
Prof. Kuprov is a world renowned magnetic resonance spectroscopy and imaging specialist with a particular focus on large-scale computer simulation of magnetic processes in chemical and biological systems; this includes quantum optimal control and machine learning methods.
Bio:
2005: DPhil, Chemistry, University of Oxford (with Prof. Peter Hore)
2005-2010: Fellow by Examination, Magdalen College, Oxford 2
007-2009: Lecturer in Chemistry, University of Durham
2009-2011: EPSRC Early Career Fellow, University of Oxford
2011-present: Associate Professor of Chemical Physics at the University of Southampton
2018-present: Associate Editor, Science Advances.
Dissolution DNP-enhanced Zero- to Ultralow-Field NMR
In session 29 held on 8th June 2021, Román Picazo-Frutos gave a talk on dissolution dynamic nuclear polarization (DNP)-enhanced zero- to ultralow-Field nuclear magnetic resonance (NMR) spectroscopy, via Zoom. The video was recorded live during the presentation and serves as an educative lecture.
Bio:
2016: Bachelor's in Physics, Universitat de Valencia, Spain
2018: Master's degree in Physics, Johannes Gutenberg Universität, Germany
2019-present: PhD student, Helmholtz-Institut, Mainz, Germany (Prof. Dmitry Budker)
Román works on Zero- and Ultra-low field Nuclear Magnetic Resonance (ZULF NMR). Román is also interested in hyperpolarization, atomic physics, photonics and relaxometry in NMR.
Google scholar: https://scholar.google.com/citations?...
Budker group website: https://budker.uni-mainz.de/
ZULF blog post: getting rid of noise source in ZULF NMR : https://blog.zulf.eu/BP3.php
Abstract:
Zero- and ultra-low field NMR (ZULF-NMR) is a modality of NMR experiment that do not require strong magnets with applications ranging from molecular spectroscopy and chemical-reaction monitoring to dark-matter searches and exotic physics. However, it suffers from low sensitivity. In this work we combine dissolution Dynamic Nuclear Polarization (dDNP) to enhance biomolecules signals in a ZULF-NMR experiment. We discuss the scope of this hyperpolarization technique for ZULF NMR, such as the sample transfer and paramagnetic relaxation caused by the polarizing agent in DNP. We believe this technique can become an established choice for hyperpolarization in ZULF NMR.
Additional references:
Proton to carbon polarization transfer in NMR: https://onlinelibrary.wiley.com/doi/a...
Observing through metal containers at zero field: https://pubs.rsc.org/en/content/artic...
General Review on ZULF NMR: https://onlinelibrary.wiley.com/doi/a...
Frequency-Agile Instrumentation for High Field DNP/EPR Spectroscopy
The 19th session of the Global NMR Discussion Meeting was held on 24th November 2020 via Zoom. Dr. Thorsten Maly gave a talk on versatile, frequency-agile instrumentation for high field dynamic nuclear polarization/electron paramagnetic resonance spectroscopy. The video was recorded live during the presentation and serves as an educative lecture. Bio: 2005 Ph.D. in Chemistry, JWG University Frankfurt, Germany (group of Prof. Thomas Prisner) 2005-2010 Postdoc, MIT, Cambridge, USA (group of Prof. Bob Griffin) 2010-present: co-founder Bridge12 Technologies, Inc., Framingham USA
Follow Thorsten on social media:
Twitter: @thmaly
Website: http://www.bridge12.com/thorsten-maly/
Google Scholar: https://scholar.google.com/citations?...
Research Gate: https://www.researchgate.net/profile/...
Abstract: To further improve the DNP methodology it is crucial to understand the EPR properties of the DNP sample at the field of the DNP experiment. However, gyrotron-based DNP system only offer limited flexibility to extract EPR parameters. In this tutorial, I will review frequency-agile instrumentation suitable for high-frequency DNP and EPR spectroscopy. I will discuss strategies for generating and detecting microwave radiation and will present the fundamental basics of quasi- optics, necessary to separate the incident from the reflected microwave beam.
Paramagnetic Metal Ion Polarizing Agents for DNP
The 15th session of the Global NMR Discussion Meeting was held on 29th September 2020 via Zoom. Prof. Björn Corzilius gave a talk on paramagnetic metal ion polarizing agents for dynamic nuclear polarization (DNP) nuclear magnetic resonance spectroscopy (NMR). The video was recorded live during the presentation and serves as an educative lecture.
Prof. Corzilius is the leader of the Solid-State DNP research group at the Institute of Chemistry and the Department of Life, Light & Matter, at the University of Rostock, Germany.
Website: http://www.solidstatednp.com/
Theoretical Understanding of MAS Dynamic Nuclear Polarization
The 14th session of the Global NMR Discussion Meeting was held on 15th September 2020 via Zoom. Dr. Asif Equbal gave a talk on the theoretical Understanding of magic angle spinning (MAS) Dynamic Nuclear Polarization (DNP). The video was recorded live during the presentation and serves as an educative lecture.
Dr. Asif Equbal is currently a postdoctoral scientist in the research group of Prof. Songi Han at the University of California Santa Barbara (UCSB), U.S.A.
Follow Dr. Equbal on Twitter: @asifequbal313
Website: https://han.chem.ucsb.edu/people/asif...
Dissolution DNP towards Clinical MRI
The 7th session of the Global NMR Discussion Meeting was held on 19th June 2020 via Zoom. Dr. Ardenkjær-Larsen gave a talk on the following topic: Dissolution DNP (Dynamic Nuclear Polarization) towards Clinical MRI (Magnetic Resonance Imaging). The video was recorded live during the presentation and serves as an educative lecture.
Prof. Ardenkjær-Larsen the head of Center for Magnetic Resonance and also the center leader for Center for Hyperpolarization in Magnetic Resonance at the Technical University of Denmark (DTU).
Follow Prof. Ardenkjær-Larsen on Twitter: @ardenkjaer
Website: cmr.healthtech.dtu.dk
Google Scholar: https://scholar.google.co.uk/citation...
DNP in Materials Science: Touching the Surface
In the fourth session of the Global NMR Discussion Meeting held on 29th May 2020 via Zoom, Dr. Pierrick Berruyer from EPFL, Lausanne (Switzerland) gave a talk on the following topic: Dynamic Nuclear Polarization Solid-State Nuclear Magnetic Resonance (NMR) Spectroscopy in Materials Science: Touching the Surface. The video was recorded live during the presentation and serves as an educative lecture for those wanting to learn about the use of Magic Angle Spinning Dynamic Nuclear Polarization for studying the surfaces of materials.
Dr. Pierrick Berruyer is currently a postdoc in Prof. Lyndon Emsley's laboratory at the Swiss Federal Institute of Technology (EPFL), Lausanne.
Follow Dr. Pierrick Berruyer on Twitter: @pierrickberruye
Dissolution Dynamic Nuclear Polarization: Genesis and Metamorphosis
In the third session of the Global NMR Discussion Meeting held on 22nd May 2020 via Zoom, Prof. Sami Jannin from CRMN, Lyon (France) gave a talk on the following topic: Dissolution Dynamic Nuclear Polarization: Genesis and Metamorphosis. The video was recorded live during the presentation and serves as an educative lecture for those wanting to learn Dissolution Dynamic Nuclear Polarization.
Prof. Sami Jannin is a professor at Université de Lyon, the Deputy Director of Center Resonance Magnétique Nucleaire À Très Hauts Champs (Lyon, France) and the Head of the Hyperpolarized Magnetic Resonance Laboratory. Research group website: https://www.hmrlab.eu/
Follow Prof. Jannin on Twitter: @Sami_HMRlab