Methyl Sidechain Probes for Solution NMR of Large Proteins
In session 25 held on 13th April 2021, Dr. Andrew McShan gave a talk on "Utility of Methyl Sidechain Probes for Solution Nuclear Magnetic Resonance (NMR) spectroscopy of large proteins", via Zoom. The video was recorded live during the presentation and serves as an educative lecture.
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Biography: Andrew received a B.S. in Biochemistry and Ph.D. in Molecular, Cellular & Developmental Biology from the University of Kansas with Roberto De Guzman. During their Ph.D., they used methyl-based solution-state NMR to characterize the structure and function of bacterial type III secretion systems. In 2016, they moved to the University of California, Santa Cruz and joined the lab of Nik Sgourakis where methyl NMR was used to elucidate mechanistic aspects of large molecular machines of the adaptive immune system. Currently, Andrew is a postdoctoral fellow at the Children’s Hospital of Philadelphia where they continue to harness the power of methyl NMR to discern the structure and function of proteins complexes of the innate immune system.
Abstract: In recent years, the use of site-specific methyl probes has pushed the molecular weight limit of solution-state NMR to beyond 1 MDa. In this tutorial, we will discuss practical aspects of methyl NMR. The first section will summarize approaches for methyl resonance assignment, including recent advances in automated assignment using methyl-methyl nuclear Overhauser effect measurements. The second section will describe methyl-based NMR methods to uncover biomolecular structure, function and dynamics. Throughout the discussion, a range of large molecular machines where methyl probes have been exploited will be highlighted, including a handful of important immunological protein complexes.