Videos
Parahydrogen-based Exchange Chemistry for Next-Generation MR
The Fall 2022 series of meetings began on September 20th with a talk by Dr. Danila Barskiy on the topic "Parahydrogen-based Exchange Chemistry for Next-Generation Magnetic Resonance". The recording serves as a tutorial.
Abstract:
In my talk, I will present our recent work demonstrating that parahydrogen-based spin chemistry can generate hyperpolarized molecules (urea, alcohols, amino acids, ammonium, glucose, etc.) for benchtop (1 tesla) NMR as well as for zero- to ultralow-field (ZULF) NMR. I will describe the basics of exchange-based hyperpolarization and discuss potential applications for chemical analysis.
Speaker's biography:
Dr. Danila A. Barskiy
PhD - Novosibirsk University, 2012 - 2015
Postdoc - Vanderbilt University, 2015 - 2017
Postdoc - University of California Berkeley, 2017 - 2020
Presently, he is a research Group Leader at the Helmholtz Institute, Johannes Gutenberg University Mainz. His group works on chemistry-oriented hyperpolarization technologies for affordable nuclear magnetic resonance (NMR) spectroscopy and imaging (MRI).
Follow Dr. Barskiy's work here:
Google scholar: https://scholar.google.com/citations?...
Website: https://budker.uni-mainz.de/?page_id=70
Twitter: https://twitter.com/tovarishbarskiy
Electrophoretic NMR: an ion speedometer for concentrated electrolytes
During the 47th session of the Global NMR Discussion Meetings held on April 19, 2022 via Zoom, Dr. David Halat from UC Berkeley gave a talk on the topic "Electrophoretic NMR: an ion speedometer for concentrated electrolytes". The recording serves as a tutorial.
Abstract: Pulsed field gradient (PFG) NMR techniques are powerful probes of self-diffusion but remain insensitive to the charged nature of the mobile species. For example, Li-ion battery electrolyte performance is strongly affected by the drift velocity of the working (Li+) cation under an applied potential, and not necessarily to its diffusivity. By synchronizing PFG pulse programs with a simultaneous electric field, electrophoretic NMR (eNMR) can selectively measure cation, anion, and solvent motion and provide a full accounting of ion transport in concentrated electrolytes. I will discuss the experimental and practical underpinnings of eNMR, as well as its complementarity to MD simulations to reveal the migration of specific solvation structures.
Speaker's biography:
2009 — 2013: B.S. Mathematics and Chemistry, Montana State University, USA.
2013 — 2018: Ph.D., University of Cambridge, UK, (with Clare P. Grey)
2018 : Postdoc and FRS Research Associate, University of Cambridge, UK (with Clare P. Grey)
2018 — present: Postdoc, University of California Berkeley, USA (with Jeffrey A. Reimer and Nitash P. Balsara)
Google Scholar: https://scholar.google.com/citations?...
Twitter: @davidhalat
Structure & Thermodynamics of Sorption Equilibria using NMR
During the 46th session of the Global NMR Discussion Meetings held on April 5, 2022 via Zoom, Prof. Vincenzo Venditti from Iowa State University gave a talk on the topic "Solution NMR methods for structural and thermodynamic investigation of sorption equilibria". The recording serves as a tutorial.
Abstract:
Nanoparticles are highly tunable materials that owe some of their unique properties to the ability to efficiently and selectively adsorb their target ligands. In this tutorial we will discuss the use of DEST, relaxation dispersion, and R1 experiments for investigating structure, kinetics, and thermodynamics of sorption with atomic resolution.
Venditti group website: https://group.chem.iastate.edu/Venditti/
Google Scholar: https://scholar.google.com/citations?...
Speaker's biography: Vincenzo Venditti received a Ph.D. from the University of Siena in 2009 for his work with Neri Niccolai and Sam Butcher on the surface accessibility of biopolymers. From 2009 to 2014 he was a Postdoctoral Fellow at the National Institutes of Health in the group of Marius Clore where he specialized in biomolecular NMR techniques for the investigation of protein structure, dynamics and interactions. Vincenzo joined the Department of Chemistry at ISU in January 2015.
Long-lived states: some unexpected applications
During the 42nd session of the Global NMR Discussion Meetings held on February 8th, 2022 via Zoom, Prof. Geoffrey Bodenhausen gave a talk on the topic "Long-lived states: some unexpected applications". The recording serves as a tutorial.
Abstract: The invention of singlet states, also known as long-lived states (LLS), by Malcolm Levitt was to be followed by many further inventions by scores of creative co-workers in Southampton and Novosibirsk: the quest for molecules immersed in environments capable of supporting singlet states with very long lifetimes, magnetization-to-singlet conversion (M2S), extremely low frequency (ELF) excitation, classification of decay rates according to the eigenvalues of the Liouvillian, effects of exchange on the lifetimes of LLS, etc. Our laboratories at EPFL and ENS have focused on a few applications of long-lived states, such as the study of slow diffusion of macromolecules, long-lived states in substrates of enzymatic reactions, long-lived states in systems with many spins, long-lived population imbalances in systems containing quadrupolar nuclei, elusive long-lived states in water (“para-water”), and long-lived states in drug molecules binding to macromolecular targets.
Speaker's biography:
1996-present: Professor, Department of Chemistry, Ecole Normale Supérieure, Paris
2001-2016: Professor, Ecole Polytechnique Fédérale de Lausanne
1985-2001: Université de Lausanne
1994-1996: National High Magnetic Field Laboratory and Florida State University, Tallahassee
1980-1985: Post-doc at ETH with Richard Ernst
1977 :D. Phil. Oxford with Ray Freeman Corresponding member of the Netherlands Academy of Sciences (KNAW), Fellow of the American Physical Society, Latsis Prize of the Swiss National Science Foundation
Follow Prof. Bodenhausen's work here:
Google scholar: https://scholar.google.fr/citations?u...
Overhauser Dynamic Nuclear Polarization in the Liquid State
During the 40th session of the Global NMR Discussion Meetings held on December 7th, 2021 via Zoom, Marcel Levien, Ph.D. student in Prof. Marina Bennati's laboratory at Max Planck, Göttingen, Germany, gave a talk on "Overhauser Dynamic Nuclear Polarization in the liquid state at room temperature at low and high magnetic fields". The recording serves as a tutorial.
Abstract: Dynamic Nuclear Polarization (DNP) is a tool that promises to overcome the long-standing sensitivity issues in modern Nuclear Magnetic Resonance (NMR). While it is already commercially available in the solid-state, in the liquid state at room temperature the development at high magnetic field is still in its infancy. However, recent advances in the understanding of the polarization transfer mechanisms and in the hardware technology demonstrate the viability of DNP in liquids at high magnetic fields. During this talk, the general Overhauser mechanism for the liquid state will be discussed. Particularly, the polarization transfer via scalar interaction, its frequency dependence as well as the role of the polarizing agent will be explained. Finally, an overview about recent applications at low field as well as developments at high magnetic field will be given.
Speaker's biography:
2012 – 2015: BSc. Chemistry, University of Göttingen, Germany
2015 – 2017: MSc. Chemistry, University of Göttingen, Germany
2018 – present: PhD student, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
What goes on inside the nucleus?
During the 39th session of the Global NMR Discussion Meetings held on November 23rd, 2021 via Zoom, Prof. Ilya Kuprov gave a talk on "What goes on inside the nucleus: origins of nuclear magnetogyric ratio and quadrupole moment". The recording serves as a tutorial.
Prof. Kuprov is a world renowned magnetic resonance spectroscopy and imaging specialist with a particular focus on large-scale computer simulation of magnetic processes in chemical and biological systems; this includes quantum optimal control and machine learning methods.
2005: DPhil, Chemistry, University of Oxford (with Prof. Peter Hore)
2005-2010: Fellow by Examination, Magdalen College, Oxford
2007-2009: Lecturer in Chemistry, University of Durham
2009-2011: EPSRC Early Career Fellow, University of Oxford
2011-present: Associate Professor of Chemical Physics at the University of Southampton
2018-present: Associate Editor, Science Advances.
Website: https://spindynamics.org
Google Scholar: https://scholar.google.com/citations?...
NMR Studies of DNA Structure and Dynamics
During the 35th session of the Global NMR Discussion Meetings on Zoom, Dr. Bharathwaj Sathyamoorthy, IISER Bhopal, India, gave a talk on the Nuclear Magnetic Resonance (NMR) studies of DNA structure and dynamics.
Abstract: Characterizing conformational preferences of biomolecules is essential to understand their function. NMR spectroscopy has been instrumental in unravelling the conformational polymorphism of DNA and sequence-specific signatures, both of which are critical for their function. This talk shall describe the journey over the past five decades towards our current understanding of DNA structure and dynamics obtained from solution-state NMR spectroscopy.
Dr. Sathyamoorthy's Biography:
2005: B.Sc. (Chemistry), University of Madras, India
2007: M.Sc. (Chemistry), Indian Institute of Technology Madras, India
2013: Ph.D. (Chemistry), State University of New York at Buffalo, USA (Prof. Thomas Szyperski)
2013-2015: Postdoctoral Research Fellow, University of Michigan/Duke University, USA (Prof. Hashim M. Al-Hashimi)
2016 – present: Assistant Professor, IISER Bhopal, India
Follow Dr. Sathyamoorthy and his work on social media:
Twitter: @bwajtweeting
Website: https://bionmr.wordpress.com
Google Scholar: https://scholar.google.com/citations?...
NMR Hardware
In Session 34 of the Global NMR Discussion Meetings, Dr. Mark Conradi gave a talk on NMR hardware.
Abstract: Many NMR spectroscopists are uncomfortable with hardware issues. This discussion will cover debugging techniques, so that malfunctions can be narrowed to particular components of the spectrometer. The testing of probes will be covered, as well as the basics of building special purpose probes. While you may not want to build your own 1.3 mm MAS probe, a probe for high-T, high-P, or in situ examination of batteries may be of interest.
Download Dr. Conradi's slides here: https://docs.google.com/presentation/... S
peaker's biography: Prof. Mark Conradi received his PhD in Physics under R.E. Norberg at Washington University in Saint Louis MO. He did a postdoctoral stint at Oak Ridge where he learned about free radicals and ESR. An assistant/ associate professorship followed at College of William and Mary in Virginia. He was a professor at Washington University for 29 years, moving five years ago to ABQMR, a small NMR contract research firm in New Mexico. Conradi's research themes included motions in molecular solids and metal-hydrogen systems and later the imaging of human lungs with hyperpolarized helium-3. Throughout, he has been interested in NMR hardware issues and techniques, such as NMR in diamond-anvil high-pressure cells and NMR in coin-cell batteries.
Google scholar: https://scholar.google.com/citations?...
Practicalities of SPINACH - a tutorial lecture
In Session 33 of the Global NMR Discussion Meetings, Prof. Ilya Kuprov gave a second talk on performing nuclear magnetic resonance (NMR) simulations using the SPINACH program, with an emphasis on practical aspects.
Watch Part 1 here: https://youtu.be/3fnRgGCKnLU
Download related resources from Prof. Kuprov: https://www.dropbox.com/sh/55kn4enfve...
Website and SPINACH download: https://spindynamics.org
Google Scholar: https://scholar.google.com/citations?...
Prof. Kuprov is a world renowned magnetic resonance spectroscopy and imaging specialist with a particular focus on large-scale computer simulation of magnetic processes in chemical and biological systems; this includes quantum optimal control and machine learning methods.
Bio:
2005: DPhil, Chemistry, University of Oxford (with Prof. Peter Hore)
2005-2010: Fellow by Examination, Magdalen College, Oxford 2
007-2009: Lecturer in Chemistry, University of Durham
2009-2011: EPSRC Early Career Fellow, University of Oxford
2011-present: Associate Professor of Chemical Physics at the University of Southampton
2018-present: Associate Editor, Science Advances.
Large-scale liquid state NMR and MRI Simulations
In Session 32 of the Global NMR Discussion Meetings, Prof. Ilya Kuprov gave a talk on "Large-scale liquid state NMR and MRI Simulations" and the inner workings of the magnetic resonance simulation software SPINACH.
Prof. Kuprov is a world renowned magnetic resonance spectroscopy and imaging specialist with a particular focus on large-scale computer simulation of magnetic processes in chemical and biological systems; this includes quantum optimal control and machine learning methods.
Website and SPINACH download: https://spindynamics.org
Google Scholar: https://scholar.google.com/citations?...
Bio:
2005: DPhil, Chemistry, University of Oxford (with Prof. Peter Hore)
2005-2010: Fellow by Examination, Magdalen College, Oxford
2007-2009: Lecturer in Chemistry, University of Durham
2009-2011: EPSRC Early Career Fellow, University of Oxford
2011-present: Associate Professor of Chemical Physics at the University of Southampton
2018-present: Associate Editor, Science Advances.
The Art of Long-Lived Nuclear Spin Order: Methods and Applications
In session 31 held on 20th July 2021, Dr. Mohamed Sabba gave a talk on "The Art of Long-Lived Nuclear Spin Order: Methods and Applications", via Zoom. The video was recorded live during the presentation and serves as an educative lecture.
Bio:
BSc at University of Greenwich with Prof. Jeremy Everett 2014-2017
PhD at University of Southampton with Prof. Malcolm Levitt 2017-2021
Postdoc with Prof. Malcolm Levitt 2021-
Follow Dr. Sabba:
Twitter: @ma_sabba
Research Gate: https://www.researchgate.net/profile/...
Abstract:
All physical systems are bound by entropy. In particular, nuclear spin order undergoes relaxation through several distinct modes. The cases of the relaxation of longitudinal and transverse order, characterized by the familiar time constants T1 and T2, are textbook examples in the field of liquid-state NMR which are routinely probed and fairly well-studied. For many years it was popularly believed that T1 was the ultimate upper bound on experimentally accessible relaxation times.
However, in coupled spin systems, it is possible to access a range of interesting configurations which had been almost completely overlooked in the formative decades of magnetic resonance. These configurations have often been organized under the loose umbrella term "long-lived nuclear spin order" owing to their exceptional lifetimes. The most prominent of these is nuclear singlet order which our group in 2004 demonstrated could be accessed easily in 2-spin-1/2 systems from ordinary room-temperature magnetization via simple pulse sequences. Since then the field has grown rapidly with insightful contributions from several excellent groups. There have been striking discoveries, such as room-temperature solution-state lifetimes beyond 1 hour in certain spin systems, which is orders of magnitude larger than T1 in the same conditions.
This talk will be an attempt at summarizing the basic theory and practice of generating, preserving, and reading out long-lived spin order in 2-spin-1/2 systems, with a pragmatic focus tailored towards the experimental spectroscopists curious in these techniques, as well as chemists interested in probing the dynamical properties of their molecules through the potentially unique and symmetry-sensitive relaxometry of long-lived order. We hope to portray this subfield of NMR as readily available rather than exotic and inscrutable. We discuss seminal experiments of the field and also present some of our recent experimental results on robust, highly efficient pulse sequences for accessing 2-spin-1/2 long-lived order in a wide range of chemical inequivalence regimes.
Two-dimensional lineshape analysis of biomolecular interactions
In session 30 held on 22nd June 2021, Dr. Chris Waudby gave a talk on Two-dimensional lineshape analysis of biomolecular interactions, via Zoom. The video was recorded live during the presentation and serves as an educative lecture. For more information on TITAN 2D NMR lineshape analysis, see https://www.nmr-titan.com.
Bio: Dr. Chris Waudby
2000-2004: BA MSci, Natural Sciences (Chemistry), University of Cambridge, UK
2004-2009: PhD, Chemistry, Prof. Chris Dobson, University of Cambridge, UK
2010-present: Postdoc, Prof. John Christodoulou, University College London, UK
Follow Dr. Waubdy: Google scholar: https://scholar.google.com/citations?...
Website: https://www.ucl.ac.uk/biosciences/dep...
Twitter: https://twitter.com/chris_waudby
Abstract: NMR titration experiments are a rich source of structural, mechanistic, thermodynamic and kinetic information on biomolecular interactions, which can be extracted through the quantitative analysis of resonance lineshapes in 2D spectra. We will review the basic principles of chemical exchange in one-dimensional NMR, and then discuss a range of surprising and useful effects that can arise when these principles are applied to multi-dimensional experiments. We will touch on the optimal design of titration experiments and selection of pulse programs, and give a tutorial on data analysis using the TITAN software package.
References:
https://www.nature.com/articles/srep2...
Dissolution DNP-enhanced Zero- to Ultralow-Field NMR
In session 29 held on 8th June 2021, Román Picazo-Frutos gave a talk on dissolution dynamic nuclear polarization (DNP)-enhanced zero- to ultralow-Field nuclear magnetic resonance (NMR) spectroscopy, via Zoom. The video was recorded live during the presentation and serves as an educative lecture.
Bio:
2016: Bachelor's in Physics, Universitat de Valencia, Spain
2018: Master's degree in Physics, Johannes Gutenberg Universität, Germany
2019-present: PhD student, Helmholtz-Institut, Mainz, Germany (Prof. Dmitry Budker)
Román works on Zero- and Ultra-low field Nuclear Magnetic Resonance (ZULF NMR). Román is also interested in hyperpolarization, atomic physics, photonics and relaxometry in NMR.
Google scholar: https://scholar.google.com/citations?...
Budker group website: https://budker.uni-mainz.de/
ZULF blog post: getting rid of noise source in ZULF NMR : https://blog.zulf.eu/BP3.php
Abstract:
Zero- and ultra-low field NMR (ZULF-NMR) is a modality of NMR experiment that do not require strong magnets with applications ranging from molecular spectroscopy and chemical-reaction monitoring to dark-matter searches and exotic physics. However, it suffers from low sensitivity. In this work we combine dissolution Dynamic Nuclear Polarization (dDNP) to enhance biomolecules signals in a ZULF-NMR experiment. We discuss the scope of this hyperpolarization technique for ZULF NMR, such as the sample transfer and paramagnetic relaxation caused by the polarizing agent in DNP. We believe this technique can become an established choice for hyperpolarization in ZULF NMR.
Additional references:
Proton to carbon polarization transfer in NMR: https://onlinelibrary.wiley.com/doi/a...
Observing through metal containers at zero field: https://pubs.rsc.org/en/content/artic...
General Review on ZULF NMR: https://onlinelibrary.wiley.com/doi/a...
Methyl Sidechain Probes for Solution NMR of Large Proteins
In session 25 held on 13th April 2021, Dr. Andrew McShan gave a talk on "Utility of Methyl Sidechain Probes for Solution Nuclear Magnetic Resonance (NMR) spectroscopy of large proteins", via Zoom. The video was recorded live during the presentation and serves as an educative lecture.
Follow Dr. McShan's work on Google scholar: https://scholar.google.com/citations?...
Biography: Andrew received a B.S. in Biochemistry and Ph.D. in Molecular, Cellular & Developmental Biology from the University of Kansas with Roberto De Guzman. During their Ph.D., they used methyl-based solution-state NMR to characterize the structure and function of bacterial type III secretion systems. In 2016, they moved to the University of California, Santa Cruz and joined the lab of Nik Sgourakis where methyl NMR was used to elucidate mechanistic aspects of large molecular machines of the adaptive immune system. Currently, Andrew is a postdoctoral fellow at the Children’s Hospital of Philadelphia where they continue to harness the power of methyl NMR to discern the structure and function of proteins complexes of the innate immune system.
Abstract: In recent years, the use of site-specific methyl probes has pushed the molecular weight limit of solution-state NMR to beyond 1 MDa. In this tutorial, we will discuss practical aspects of methyl NMR. The first section will summarize approaches for methyl resonance assignment, including recent advances in automated assignment using methyl-methyl nuclear Overhauser effect measurements. The second section will describe methyl-based NMR methods to uncover biomolecular structure, function and dynamics. Throughout the discussion, a range of large molecular machines where methyl probes have been exploited will be highlighted, including a handful of important immunological protein complexes.
Low-field vs high-field NMR: why not both?
In session 24, held virtually via zoom on 16th March 2021 Dr. Fabien Ferrage gave a talk on the topic "Low-field vs. high-field nuclear magnetic resonance spectroscopy: why not both?". The video was recorded live during the presentation and serves as an educative lecture.
Dr. Ferrage is currently Director of research at CNRS and associate professor at Ecole Normale Supérieure (ENS), Paris, France.
Website: http://www.paris-en-resonance.fr/CvHt...
Twitter: @fabferrage
Google scholar: https://scholar.google.fr/citations?u...
Abstract: High magnetic fields usually provide higher sensitivity and resolution, which are essential to investigate complex systems by NMR. Low-field NMR is cheap, convenient, and sometimes even better than high-field NMR. I will discuss and compare both approaches in the first part of the seminar. In the second part, I will introduce two-field NMR, in which we combine high- and low-field NMR in a single experiment. I will present how two-field NMR works and can lead to better NMR spectra in challenging systems.
Parahydrogen hyperpolarization: biomedical imaging to RASER physics
After a brief break during December 2020, Global NMR Discussion Meetings is excited to continue hosting educative lectures on a wide variety of NMR topics starting January 2021. In session 20 that was held on 19th January, 2021 (via Zoom), Prof. Thomas Theis gave a talk on "Parahydrogen based hyperpolarization, novel development for applications ranging from biomedical imaging to RASER physics." The video was recorded live during the presentation and serves as an educative lecture.
Bio: Dr. Thomas Theis is Assistant Professor at North Carolina State University and Adjunct Assistant Professor at the University of North Carolina at Chapel Hill. His research is focused on hyperpolarization technology and unconventional NMR and MRI detection schemes. Dr. Theis was born in Heidelberg, Germany, raised in Tenerife, Spain, and completed his undergraduate and masters at the Georg-August University of Goettingen (Germany). Theis received his PhD in 2012 from UC Berkeley (USA) working with Prof. Alexander Pines on "zero-field NMR" and “parahydrogen hyperpolarization schemes” for portable NMR. Dr. Theis conducted postdoctoral research at Duke University (USA) with Prof. Warren Warren focused on "singlet states for hyperpolarization storage", and worked on "low-field NMR" as visiting professor at RWTH Aachen University with Prof. Stephan Appelt (Germany). In 2015, he was promoted to Research Assistant Professor at Duke University developing “cost-efficient hyperpolarization techniques for molecular imaging”. Since 2018, Dr. Theis leads the North Carolina State Hyperpolarization Laboratory.
Follow Prof. Theis on:
Google scholar: https://scholar.google.com/citations?...
Research group website: https://theislab.wordpress.ncsu.edu/p...
Abstract: Parahydrogen based hyperpolarization methods can create nuclear spin hyperpolarization directly in room temperature solutions to enhance NMR and MRI signals by up to seven orders of magnitude, depending on the magnetic field. Novel approaches to Parahydrogen Induced Polarization (PHIP) open new windows of opportunity in the field of magnetic resonance. In this talk the four following topics are discussed:
1) Parahydrogen as a Source of Spin Order, an Introduction.
2) Optical detection of PHIP with Rb-vapor and NV-centers.
3) Everlasting Sources of Long-Lasting Hyperpolarization.
4) The parahydrogen pumped RASER for precision measurements.
Nanoparticle-assisted NMR relaxation in protein dynamics & metabolomics
The 18th session of the Global NMR Discussion Meeting was held on 10th November 2020 via Zoom. Dr. Mouzhe Xie gave a talk on nanoparticle-assisted nuclear magnetic resonance (NMR) relaxation in protein dynamics and metabolomics. The video was recorded live during the presentation and serves as an educative lecture.
Bio: Mouzhe Xie studied chemical biology at Xiamen University (China) from 2009-2013. He received his Ph.D. degree from The Ohio State University (USA) in 2018, where he developed and applied solution NMR spectroscopy to study protein dynamics and metabolomics. He then spent 5 months at EPFL (Switzerland) as a visiting scientist. Currently, he is conducting postdoctoral research on nanoscale NMR and quantum sensing at the University of Chicago (USA).
Personal website: https://sites.google.com/view/xiemouzhe
Google scholar: https://scholar.google.com/citations?...
LinkedIn: https://www.linkedin.com/in/mouzhe-xi...
Abstract: In nanoparticle-assisted solution NMR, synthetic nanoparticles are added to NMR samples, which reduce the reorientational motion of molecules or protein regions in a selective or differential way. This leads to interesting spectral observations that contain critical information about the physicochemical properties, structures, dynamics, and functions of biomolecules. In this tutorial, I will introduce the basics of NMR relaxation theories, followed by some technical details including pulse selection and data processing. The discussion will be buttressed by recent studies on some important topics, such as supra-τc (slow) internal motions of globular proteins, cooperative binding of intrinsically disordered proteins to inorganic surfaces, and accurate metabolite identification in the context of NMR-based metabolomics."
Protein Structure Determination Using Paramagnetic NMR
The 17th session of the Global NMR Discussion Meeting was held on 27th October 2020 via Zoom. Dr. Alireza Bahramzadeh gave a talk on the use of paramagnetic nuclear magnetic resonance (NMR) spectroscopy for protein structure determination. The video was recorded live during the presentation and serves as an educative lecture.
Abstract: The long-range nature of the paramagnetic effects arising from unpaired electrons of metal ions renders them a powerful NMR spectroscopic tool for the study of the structure and dynamics of biological macromolecules. In this tutorial, we will discuss protein structure determination using paramagnetic NMR. The first part will cover different types of paramagnetic metal ions and their paramagnetic effects in NMR, mainly focusing on using techniques which better immobilize metal ions onto the proteins and do not impact the protein structure; the second part will consider Pseudocontact Shifts (PCSs) for determining the 3D structure of proteins.
Biography: Alireza received a bachelor of Chemical Engineering and a master of Polymer engineering from the University of Tehran, Iran. During his master’s project, he worked on developing new nanomembranes for heavy metal ions removal in water. In 2015, he moved to Australia and joined the research group of Professor Gottfried Otting to undertake a Ph.D. During his Ph.D., he worked on developing new ways of studying protein structure using paramagnetic NMR spectroscopy.
Follow Alireza on Twitter: @alireza_bahramz
Google scholar page: https://scholar.google.com/citations?...
Research Gate: https://www.researchgate.net/profile/...